Hotspot

rpl32-trnL-UAG

SSC · 115231-116178 · 948 bp

Back to Ophiopogon

Pi 0.00729

MarkerSeek score 65.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 948
variable_sites: 12
pi: 0.00729
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAACAAATAAGA	AGCGTGTCTACCGATTTCAC	35.0	50.0	53.8	58.0	748-1011 bp	1.0	51.2
rpl32-trnL-UAG_p2	TGTGCGACAAACAAATAAGA	CAGCGTGTCTACCGATTTCA	35.0	50.0	53.8	58.0	749-1012 bp	1.0	51.2
rpl32-trnL-UAG_p3	TGTGCGACAAACAAATAAGA	TTTACAGTATGCCGCCACTC	35.0	50.0	53.8	58.0	808-1071 bp	1.0	51.0
rpl32-trnL-UAG_p4	TGTGCGACAAACAAATAAGA	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	53.8	57.9	758-1021 bp	1.0	50.2
rpl32-trnL-UAG_p5	TGTGCGACAAACAAATAAGAA	AGCGTGTCTACCGATTTCAC	33.3	50.0	54.6	58.0	748-1011 bp	1.0	50.1