Hotspot

ndhF-rpl32

SSC · 114083-115056 · 974 bp

Back to Ophiopogon

Pi 0.011

MarkerSeek score 65.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 974
variable_sites: 25
pi: 0.011
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAATAAGCA	ACTGACTTTGCTAAAGAAAAAGCT	45.0	33.3	57.8	57.5	1000-1186 bp	1.0	48.7
ndhF-rpl32_p2	TGTTGCTGTCGGAATAAGCA	ACTGACTTTGCTAAAGAAAAAGC	45.0	34.8	57.8	56.2	1000-1186 bp	1.0	46.0
ndhF-rpl32_p3	TGCTGTCGGAATAAGCAGAA	ACTGACTTTGCTAAAGAAAAAGCT	45.0	33.3	57.2	57.5	997-1183 bp	1.0	45.8
ndhF-rpl32_p4	TTGCTGTCGGAATAAGCAGA	ACTGACTTTGCTAAAGAAAAAGCT	45.0	33.3	57.2	57.5	998-1184 bp	1.0	45.8
ndhF-rpl32_p5	TGTTGCTGTCGGAATAAGCA	CTGACTTTGCTAAAGAAAAAGCT	45.0	34.8	57.8	55.9	999-1185 bp	1.0	45.8