Hotspot

rps3-rpl22

LSC · 84939-85007 · 69 bp

Back to Ophiopogon

Pi 0.0778

MarkerSeek score 83.30

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 9
pi: 0.0778
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CCAAGTCTGAAACCAAGTGGA	TTAAGACCCAGGGCTAGAGG	47.6	55.0	58.1	57.8	178-197 bp	1.0	77.8
rps3-rpl22_p2	TGGTTGTGTGAACCAAAAGGA	TTAAGACCCAGGGCTAGAGG	42.9	55.0	58.2	57.8	218-237 bp	1.0	76.2
rps3-rpl22_p3	ACCAAGTCTGAAACCAAGTGG	TTAAGACCCAGGGCTAGAGG	47.6	55.0	58.3	57.8	179-198 bp	1.0	75.5
rps3-rpl22_p4	TGGTTGTGTGAACCAAAAGG	TTAAGACCCAGGGCTAGAGG	45.0	55.0	56.7	57.8	218-237 bp	1.0	75.0
rps3-rpl22_p5	CCAAGTCTGAAACCAAGTGG	TTAAGACCCAGGGCTAGAGG	50.0	55.0	56.5	57.8	178-197 bp	1.0	74.7