Hotspot

psaJ-rpl33

LSC · 68597-69117 · 521 bp

Back to Ophiopogon

Pi 0.0129

MarkerSeek score 65.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 16
pi: 0.0129
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATATCTTTCCACAGCGCCTG	GCCTGGCGATTCCTTATTGA	50.0	50.0	58.0	58.0	720-736 bp	1.0	82.3
psaJ-rpl33_p2	ATATCTTTCCACAGCGCCTG	TAGAAATGCCTGGCGATTCC	50.0	50.0	58.0	58.0	727-743 bp	1.0	82.3
psaJ-rpl33_p3	CATATCTTTCCACAGCGCCT	GCCTGGCGATTCCTTATTGA	50.0	50.0	58.0	58.0	721-737 bp	1.0	82.3
psaJ-rpl33_p4	CATATCTTTCCACAGCGCCT	TAGAAATGCCTGGCGATTCC	50.0	50.0	58.0	58.0	728-744 bp	1.0	82.2
psaJ-rpl33_p5	ATGGTTTGGGTCTTTAGCGG	GCCTGGCGATTCCTTATTGA	50.0	50.0	58.2	58.0	687-703 bp	1.0	81.4