Hotspot

accD-psaI

LSC · 59995-60386 · 392 bp

Back to Ophiopogon

Pi 0.0208

MarkerSeek score 68.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 17
pi: 0.0208
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAATCGTACCGCGTAATCT	AAAGAAGCCATTGCAATCGC	50.0	45.0	57.9	58.0	554-586 bp	1.0	85.3
accD-psaI_p2	CCAATCGTACCGCGTAATCT	GCAGGAAATACTAGGCCCAC	50.0	55.0	57.9	58.0	536-568 bp	1.0	85.2
accD-psaI_p3	AGTTATTTCAGCTGCACGGT	AAAGAAGCCATTGCAATCGC	45.0	45.0	57.8	58.0	514-546 bp	1.0	85.1
accD-psaI_p4	AGTTATTTCAGCTGCACGGT	GCAGGAAATACTAGGCCCAC	45.0	55.0	57.8	58.0	496-528 bp	1.0	84.9
accD-psaI_p5	ATTTCAGCTGCACGGTTTCT	AAAGAAGCCATTGCAATCGC	45.0	45.0	58.4	58.0	510-542 bp	1.0	83.4