Pi
0.0125
MarkerSeek score
66.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 987-1094 bp | 1.0 | 84.1 |
| trnT-UGU-trnL-UAA_p2 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 995-1102 bp | 1.0 | 83.5 |
| trnT-UGU-trnL-UAA_p3 | AAATGCGATGCTCTAACCCC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.3 | 58.0 | 959-1066 bp | 1.0 | 83.5 |
| trnT-UGU-trnL-UAA_p4 | AAATGCGATGCTCTAACCCC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.3 | 58.1 | 967-1074 bp | 1.0 | 83.0 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ATCCAATCAAGTCCGTAGCG | 50.0 | 50.0 | 57.8 | 57.8 | 1010-1117 bp | 1.0 | 82.3 |