Hotspot

psbC-trnS-UGA

LSC · 35546-35693 · 148 bp

Back to Ophiopogon

Pi 0.0254
MarkerSeek score 68.80
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
148
variable_sites
9
pi
0.0254
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GGTGGCTGAGTGGTTGATAG 55.0 55.0 58.2 58.0 253-262 bp 1.0 84.1
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GGTTGATAGCTCCGGTCTTG 55.0 55.0 58.2 58.1 242-251 bp 1.0 84.1
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC GCTGAGTGGTTGATAGCTCC 55.0 55.0 58.2 58.1 249-258 bp 1.0 84.0
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC GGCTGAGTGGTTGATAGCTC 55.0 55.0 58.2 58.1 250-259 bp 1.0 84.0
psbC-trnS-UGA_p5 TTCCATGACCCCTCTTAGCT GGTGGCTGAGTGGTTGATAG 50.0 55.0 57.7 58.0 245-254 bp 1.0 83.4