Hotspot

psbM-trnD-GUC

LSC · 30464-31190 · 727 bp

Back to Ophiopogon

Pi 0.00983

MarkerSeek score 62.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 17
pi: 0.00983
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGCTATACACCTTGCTGGGA	45.0	50.0	55.8	58.4	852-865 bp	1.0	61.9
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	826-839 bp	1.0	61.5
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	ACACCTTGCTGGGATTGTAG	45.0	50.0	55.8	57.5	846-859 bp	1.0	61.3
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	45.0	50.0	55.8	57.5	845-858 bp	1.0	61.3
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	ATGCTATACACCTTGCTGGG	45.0	50.0	55.8	57.4	853-866 bp	1.0	60.1