Hotspot

psbI-trnS-GCU

LSC · 8220-8344 · 125 bp

Back to Ophiopogon

Pi 0.0163

MarkerSeek score 62.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 5
pi: 0.0163
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CTTATCTAACGATCCGGGCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	57.6	58.2	185-191 bp	1.0	80.0
psbI-trnS-GCU_p2	CTTATCTAACGATCCGGGCC	ATTGGGAGAGATGGCTGAGT	55.0	50.0	57.6	58.1	254-260 bp	1.0	79.6
psbI-trnS-GCU_p3	CTTATCTAACGATCCGGGCC	TTCGAATCCCTCTCTCTCCG	55.0	55.0	57.6	58.3	182-188 bp	1.0	79.3
psbI-trnS-GCU_p4	CTTATCTAACGATCCGGGCC	GTGGACTAAAGCGGCAGATT	55.0	50.0	57.6	58.3	236-242 bp	1.0	78.7
psbI-trnS-GCU_p5	CTTATCTAACGATCCGGGCC	TGGACTAAAGCGGCAGATTG	55.0	50.0	57.6	58.3	235-241 bp	1.0	78.7