Hotspot

rps8

Genome · 92912-93310 · 399 bp

Back to Ophioglossum

Pi 0.00167

MarkerSeek score 39.80

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: Genome
length_bp: 399
variable_sites: 1
pi: 0.00167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	GGGAGGAATTTAGCGGTGAA	ATGCGTGAAGATTATGGGGG	50.0	50.0	57.6	57.7	536 bp	1.0	74.6
rps8_p2	AGGGAGGAATTTAGCGGTGA	ATGCGTGAAGATTATGGGGG	50.0	50.0	58.4	57.7	537 bp	1.0	74.6
rps8_p3	AAGGGAGGAATTTAGCGGTG	ATGCGTGAAGATTATGGGGG	50.0	50.0	57.6	57.7	538 bp	1.0	74.6
rps8_p4	GGGAGGAATTTAGCGGTGAA	TGGGGGTAGGGTGATAGAGA	50.0	55.0	57.6	58.4	522 bp	1.0	73.9
rps8_p5	AGGGAGGAATTTAGCGGTGA	TGGGGGTAGGGTGATAGAGA	50.0	55.0	58.4	58.4	523 bp	1.0	73.8