Hotspot

rpoB

Genome · 37717-40905 · 3189 bp

Back to Ophioglossum

Pi 0.000209

MarkerSeek score 29.60

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: Genome
length_bp: 3189
variable_sites: 1
pi: 0.000209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	ACCACCTTATGCATGGTTTG	GATCGGGACAAATTGGTCGA	45.0	50.0	56.3	58.0	262 bp	1.0	66.4
rpoB_p2	ACCACCTTATGCATGGTTTG	TCAACAAAGGTGTGGATCGG	45.0	50.0	56.3	58.1	276 bp	1.0	65.7
rpoB_p3	ACCACCTTATGCATGGTTTG	TGGATCGGGACAAATTGGTC	45.0	50.0	56.3	57.9	264 bp	1.0	65.6
rpoB_p4	ACCACCTTATGCATGGTTTG	GGTGTGGATCGGGACAAATT	45.0	50.0	56.3	58.2	268 bp	1.0	65.2
rpoB_p5	ACCACCTTATGCATGGTTTG	GTGGATCGGGACAAATTGGT	45.0	50.0	56.3	58.2	265 bp	1.0	65.2