Hotspot

rpl2

Genome · 96918-98432 · 1515 bp

Back to Ophioglossum

Pi 0.00044

MarkerSeek score 46.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: Genome
length_bp: 1515
variable_sites: 1
pi: 0.00044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TCTCTCCTCTTGCGTTGAGA	TTGTTACACTCCGAACTGGC	50.0	50.0	58.1	58.1	1709 bp	1.0	78.3
rpl2_p2	GGCTACGAAAGGACCCTTTT	TTGTTACACTCCGAACTGGC	50.0	50.0	57.8	58.1	1663 bp	1.0	77.5
rpl2_p3	CTCTCTCCTCTTGCGTTGAG	TTGTTACACTCCGAACTGGC	55.0	50.0	57.7	58.1	1710 bp	1.0	76.8
rpl2_p4	TCTCTCCTCTTGCGTTGAGA	ACACTCCGAACTGGCTATTC	50.0	50.0	58.1	57.3	1704 bp	1.0	73.9
rpl2_p5	GGCTACGAAAGGACCCTTTT	ACACTCCGAACTGGCTATTC	50.0	50.0	57.8	57.3	1658 bp	1.0	73.1