Hotspot

ccsA

Genome · 112462-113415 · 954 bp

Back to Ophioglossum

Pi 0.000699

MarkerSeek score 46.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 954
variable_sites: 1
pi: 0.000699
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAAGGTACGACCCTAGCCTT	TTGGTACCAGACGAACAATC	50.0	45.0	58.1	55.4	1054 bp	1.0	58.9
ccsA_p2	AAAGGTACGACCCTAGCCTT	TTTGGTACCAGACGAACAATC	50.0	42.9	58.1	56.2	1055 bp	1.0	57.2
ccsA_p3	CCTAGGTTCAGGTGTGCAAA	TTGGTACCAGACGAACAATC	50.0	45.0	57.7	55.4	1090 bp	1.0	57.2
ccsA_p4	CCTAGGTTCAGGTGTGCAAA	TTTGGTACCAGACGAACAATC	50.0	42.9	57.7	56.2	1091 bp	1.0	55.5
ccsA_p5	AAGGTACGACCCTAGCCTTC	TTGGTACCAGACGAACAATC	55.0	45.0	58.5	55.4	1053 bp	1.0	55.2