Hotspot

rpl20-rps12

Genome · 81585-82229 · 645 bp

Back to Ophioglossum

Pi 0.00103

MarkerSeek score 46.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: Genome
length_bp: 645
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATACATCCACGT	TAGTGGGAGCGATTCCTCAT	50.0	50.0	58.0	57.9	719 bp	1.0	79.2
rpl20-rps12_p2	TTACGACGTCTCCGAGCTAT	TAGTGGGAGCGATTCCTCAT	50.0	50.0	58.1	57.9	729 bp	1.0	79.0
rpl20-rps12_p3	TACTGTTACGACGTCTCCGA	TAGTGGGAGCGATTCCTCAT	50.0	50.0	57.9	57.9	734 bp	1.0	78.8
rpl20-rps12_p4	TCCGAGCTATACATCCACGT	GGAGCGATTCCTCATTCCTC	50.0	55.0	58.0	57.8	714 bp	1.0	78.6
rpl20-rps12_p5	TTACGACGTCTCCGAGCTAT	GGAGCGATTCCTCATTCCTC	50.0	55.0	58.1	57.8	724 bp	1.0	78.4