Hotspot

trnL-CAA-ycf2

Genome · 4572-6149 · 1578 bp

Back to Ophioglossum

Pi 0.00591

MarkerSeek score 55.50

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: Genome
length_bp: 1578
variable_sites: 14
pi: 0.00591
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	ACAGCTTGGAGGTTCGAATC	TGAAGATATCTTTTCCGGTTGA	50.0	36.4	57.9	55.2	1632-1912 bp	1.0	45.3
trnL-CAA-ycf2_p2	ATTGAGTGCCTTGATGGTGG	TGAAGATATCTTTTCCGGTTGA	50.0	36.4	58.2	55.2	1688-1968 bp	1.0	44.8
trnL-CAA-ycf2_p3	CACAGCTTGGAGGTTCGAAT	TGAAGATATCTTTTCCGGTTGA	50.0	36.4	58.2	55.2	1633-1913 bp	1.0	44.6
trnL-CAA-ycf2_p4	AACGGTAAACACGCGAGATT	TGAAGATATCTTTTCCGGTTGA	45.0	36.4	58.2	55.2	1668-1948 bp	1.0	44.6
trnL-CAA-ycf2_p5	CGGAATGGAATTGAGTGCCT	TGAAGATATCTTTTCCGGTTGA	50.0	36.4	58.2	55.2	1697-1977 bp	1.0	44.6