Hotspot

rps16

LSC · 5146-6260 · 1115 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1115
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGAATAGAGCAAGAAAAGAAGG	TCTCTGTACCGACTGAACCA	39.1	50.0	56.9	57.7	1301 bp	1.0	44.8
rps16_p2	ACGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	37.5	50.0	58.2	57.7	1301 bp	1.0	44.1
rps16_p3	CGAATAGAGCAAGAAAAGAAGGA	TCTCTGTACCGACTGAACCA	39.1	50.0	56.6	57.7	1300 bp	1.0	42.8
rps16_p4	ACGAATAGAGCAAGAAAAGAAGG	GTCTCTGTACCGACTGAACC	39.1	55.0	56.9	57.4	1302 bp	1.0	42.0
rps16_p5	ACGAATAGAGCAAGAAAAGAAGGA	GTCTCTGTACCGACTGAACC	37.5	55.0	58.2	57.4	1302 bp	1.0	41.3