Hotspot

trnE-UUC-rpoB

LSC · 12032-12942 · 911 bp

Back to Onopordum

Pi 0

MarkerSeek score 40.00

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	AACCCTCGTATTCTCCGTGA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	1046 bp	1.0	61.0
trnE-UUC-rpoB_p2	ACCCTCGTATTCTCCGTGAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	1045 bp	1.0	61.0
trnE-UUC-rpoB_p3	TGAACCCTCGTATTCTCCGT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	1048 bp	1.0	61.0
trnE-UUC-rpoB_p4	GAACCCTCGTATTCTCCGTG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	57.8	56.8	1047 bp	1.0	60.6
trnE-UUC-rpoB_p5	ACCCTCGTATTCTCCGTGAA	TCAAACCCTGATCAATGAACCT	50.0	40.9	58.2	57.6	1035 bp	1.0	59.7