Hotspot

psbM-trnD-GUC

LSC · 10927-11601 · 675 bp

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Pi 0

MarkerSeek score 58.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAATGAACAGAGCAGTAGCA	TAGATCCCGTCTGATTCCCC	40.0	55.0	55.0	58.0	815-816 bp	1.0	55.9
psbM-trnD-GUC_p2	AATGAACAGAGCAGTAGCAA	TAGATCCCGTCTGATTCCCC	40.0	55.0	55.0	58.0	814-815 bp	1.0	55.9
psbM-trnD-GUC_p3	AAATGAACAGAGCAGTAGCA	TCTGATTCCCCTGGGATTGT	40.0	50.0	55.0	58.0	806-807 bp	1.0	55.8
psbM-trnD-GUC_p4	AATGAACAGAGCAGTAGCAA	TCTGATTCCCCTGGGATTGT	40.0	50.0	55.0	58.0	805-806 bp	1.0	55.8
psbM-trnD-GUC_p5	AAATGAACAGAGCAGTAGCAA	TAGATCCCGTCTGATTCCCC	38.1	55.0	55.8	58.0	815-816 bp	1.0	54.4