Hotspot

ycf1

Genome · 118071-123170 · 5100 bp

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Pi 0.000392

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5100
variable_sites: 2
pi: 0.000392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACATGCGGCCAAAAGTTTT	TTGAGCAGCTAACAGGTTCG	40.0	50.0	57.7	58.2	268 bp	1.0	76.1
ycf1_p2	AACATGCGGCCAAAAGTTTT	GGGGCCGAAAAATCGTTTAC	40.0	50.0	57.7	57.7	248 bp	1.0	75.6
ycf1_p3	CAACATGCGGCCAAAAGTTT	TTGAGCAGCTAACAGGTTCG	45.0	50.0	58.4	58.2	269 bp	1.0	75.3
ycf1_p4	AACATGCGGCCAAAAGTTTT	CTACCCGTTGAGCAGCTAAC	40.0	55.0	57.7	58.4	275 bp	1.0	74.8
ycf1_p5	CAACATGCGGCCAAAAGTTT	GGGGCCGAAAAATCGTTTAC	45.0	50.0	58.4	57.7	249 bp	1.0	74.8