Hotspot

rpoC1

Genome · 20521-23322 · 2802 bp

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Pi 0

MarkerSeek score 56.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2802
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCATTATAGAAACCTCACCTTGA	TGGAGAGGGAGAGTGAAAACA	34.8	47.6	54.8	58.3	2995-2998 bp	1.0	40.1
rpoC1_p2	TCATTATAGAAACCTCACCTTGA	GGAGAGGGAGAGTGAAAACA	34.8	50.0	54.8	56.5	2994-2997 bp	1.0	40.1
rpoC1_p3	TCATTATAGAAACCTCACCTTGA	TGGAGAGGGAGAGTGAAAAC	34.8	50.0	54.8	56.5	2995-2998 bp	1.0	40.1
rpoC1_p4	TCATTATAGAAACCTCACCTTGAT	TGGAGAGGGAGAGTGAAAACA	33.3	47.6	55.3	58.3	2995-2998 bp	1.0	40.1
rpoC1_p5	TCATTATAGAAACCTCACCTTGA	TTGGAGAGGGAGAGTGAAAAC	34.8	47.6	54.8	57.2	2996-2999 bp	1.0	40.1