Hotspot

atpH

Genome · 13320-13565 · 246 bp

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Pi 0.00407

MarkerSeek score 43.50

Primer available yes

Label atpH

Feature Metadata

label_name: atpH
feature_type: gene
region: Genome
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH_p1	CTACTCCCCGCTACAAACAG	CGTTGCGCCGATTCTAGTTA	55.0	50.0	57.7	58.4	343 bp	1.0	74.3
atpH_p2	CTACTCCCCGCTACAAACAG	TAGAAAGTCTTCGTTGCGCC	55.0	50.0	57.7	58.6	354 bp	1.0	73.3
atpH_p3	CTACTCCCCGCTACAAACAG	CTAGAAAGTCTTCGTTGCGC	55.0	50.0	57.7	57.2	355 bp	1.0	71.7
atpH_p4	CTACTCCCCGCTACAAACAG	TTGCGCCGATTCTAGTTAGT	55.0	45.0	57.7	57.0	341 bp	1.0	70.2
atpH_p5	GCTACTCCCCGCTACAAAC	CGTTGCGCCGATTCTAGTTA	57.9	50.0	57.9	58.4	344 bp	1.0	68.2