Hotspot

matK-rps16

Genome · 2917-3532 · 616 bp

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Pi 0.00649

MarkerSeek score 62.50

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 616
variable_sites: 4
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CGCTAAGTCCTTCTTCCTGG	ATTTTGAGACGGGCGAAGTT	55.0	45.0	57.7	58.1	743-811 bp	1.0	77.1
matK-rps16_p2	CGCTAAGTCCTTCTTCCTGG	GGGCGCAATCAACAAAAACT	55.0	45.0	57.7	58.1	772-840 bp	1.0	76.9
matK-rps16_p3	CGCTAAGTCCTTCTTCCTGG	TGAACAAGTCGGGATCAACC	55.0	50.0	57.7	57.8	720-788 bp	1.0	76.6
matK-rps16_p4	CGCTAAGTCCTTCTTCCTGG	TAAGAAAGGGGCGCAATCAA	55.0	45.0	57.7	57.8	780-848 bp	1.0	76.3
matK-rps16_p5	CGCTAAGTCCTTCTTCCTGG	TTAAGAAAGGGGCGCAATCA	55.0	45.0	57.7	57.8	781-849 bp	1.0	76.3