Hotspot

tRNA-Asp-tRNA-Tyr

Genome · 112940-113366 · 427 bp

Back to Onobrychis

Pi 0.303
MarkerSeek score 73.30
Primer available yes
Label tRNA-Asp-tRNA-Tyr

Feature Metadata

label_name
tRNA-Asp-tRNA-Tyr
feature_type
igs
region
Genome
length_bp
427
variable_sites
91
pi
0.303
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.44
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Asp-tRNA-Tyr_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 499 bp 0.5 58.3
tRNA-Asp-tRNA-Tyr_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 498 bp 0.5 58.3
tRNA-Asp-tRNA-Tyr_p3 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 520 bp 0.5 57.4
tRNA-Asp-tRNA-Tyr_p4 CGGTGCTCTGACCAATTGAA GGGACGGACTGTAAATTCGT 50.0 50.0 58.5 57.6 519 bp 0.5 57.2
tRNA-Asp-tRNA-Tyr_p5 CGGTGCTCTGACCAATTGAA GATTCTTCCTGGGTCGATGC 50.0 55.0 58.5 58.4 553 bp 0.5 56.9