Hotspot

tRNA-Ser-tRNA-Gly

Genome · 91923-92631 · 709 bp

Back to Onobrychis

Pi 0.253

MarkerSeek score 81.10

Primer available yes

Label tRNA-Ser-tRNA-Gly

Feature Metadata

label_name: tRNA-Ser-tRNA-Gly
feature_type: igs
region: Genome
length_bp: 709
variable_sites: 270
pi: 0.253
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Gly_p1	GCTTTAGTCCACTCAGCCAT	ACGAATCGCACTTTTACCACT	50.0	42.9	57.6	58.2	768-769 bp	0.5	47.2
tRNA-Ser-tRNA-Gly_p2	GCTTTAGTCCACTCAGCCAT	ACGAATCGCACTTTTACCAC	50.0	45.0	57.6	56.8	768-769 bp	0.5	47.2
tRNA-Ser-tRNA-Gly_p3	GCTTTAGTCCACTCAGCCAT	TCCATAGAACGAATCGCACT	50.0	45.0	57.6	56.8	776-777 bp	0.5	47.1
tRNA-Ser-tRNA-Gly_p4	AGTCCACTCAGCCATCTCTC	ACGAATCGCACTTTTACCACT	55.0	42.9	58.5	58.2	763-764 bp	0.5	46.4
tRNA-Ser-tRNA-Gly_p5	AGTCCACTCAGCCATCTCTC	ACGAATCGCACTTTTACCAC	55.0	45.0	58.5	56.8	763-764 bp	0.5	46.3