Hotspot

tRNA-Gln-tRNA-Ser

Genome · 90531-91835 · 1305 bp

Back to Onobrychis

Pi 0.273

MarkerSeek score 81.00

Primer available yes

Label tRNA-Gln-tRNA-Ser

Feature Metadata

label_name: tRNA-Gln-tRNA-Ser
feature_type: igs
region: Genome
length_bp: 1305
variable_sites: 538
pi: 0.273
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gln-tRNA-Ser_p1	ACTTTCTGGTTCCGGTTCAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.7	58.0	2078-2081 bp	0.5	58.0
tRNA-Gln-tRNA-Ser_p2	CTTTCTGGTTCCGGTTCAGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.7	58.0	2077-2080 bp	0.5	58.0
tRNA-Gln-tRNA-Ser_p3	ACTTTCTGGTTCCGGTTCAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.7	58.0	2079-2082 bp	0.5	58.0
tRNA-Gln-tRNA-Ser_p4	CTTTCTGGTTCCGGTTCAGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.7	58.0	2078-2081 bp	0.5	58.0
tRNA-Gln-tRNA-Ser_p5	CTTTCTGGTTCCGGTTCAGT	CTAGCTGGGCTCTTGTTCTG	50.0	55.0	57.7	58.0	2302-2306 bp	0.5	57.9