Hotspot

tRNA-Trp-tRNA-Gln

Genome · 81799-90457 · 8659 bp

Back to Onobrychis

Pi 0.246

MarkerSeek score 71.90

Primer available yes

Label tRNA-Trp-tRNA-Gln

Feature Metadata

label_name: tRNA-Trp-tRNA-Gln
feature_type: igs
region: Genome
length_bp: 8659
variable_sites: 3221
pi: 0.246
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Trp-tRNA-Gln_p1	CCACACGAACTTGGGAAAGA	ATTTCCGGCAATTGCAATGG	50.0	45.0	58.0	58.0	772 bp	0.5	59.6
tRNA-Trp-tRNA-Gln_p2	CTTCCACACGAACTTGGGAA	ATTTCCGGCAATTGCAATGG	50.0	45.0	58.0	58.0	775 bp	0.5	59.6
tRNA-Trp-tRNA-Gln_p3	TCCACACGAACTTGGGAAAG	ATTTCCGGCAATTGCAATGG	50.0	45.0	58.0	58.0	773 bp	0.5	59.6
tRNA-Trp-tRNA-Gln_p4	ATCAAGAGGGGTCAAGGGAA	ATTTCCGGCAATTGCAATGG	50.0	45.0	57.9	58.0	876 bp	0.5	59.5
tRNA-Trp-tRNA-Gln_p5	TCAAGAGGGGTCAAGGGAAT	ATTTCCGGCAATTGCAATGG	50.0	45.0	57.9	58.0	875 bp	0.5	59.5