Hotspot

tRNA-Leu-tRNA-Pro

Genome · 62261-81470 · 19210 bp

Back to Onobrychis

Pi 0.248
MarkerSeek score 71.70
Primer available yes
Label tRNA-Leu-tRNA-Pro

Feature Metadata

label_name
tRNA-Leu-tRNA-Pro
feature_type
igs
region
Genome
length_bp
19210
variable_sites
7177
pi
0.248
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.75
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu-tRNA-Pro_p1 TCGACTTCGACTCGCTTTTT GGTTCATGGCCAAGGGTAAA 45.0 50.0 57.9 58.1 780 bp 0.5 58.4
tRNA-Leu-tRNA-Pro_p2 TCGACTTCGACTCGCTTTTT AAAGGAGGTTCATGGCCAAG 45.0 50.0 57.9 58.1 786 bp 0.5 58.4
tRNA-Leu-tRNA-Pro_p3 TAACTCCGGGAAGGTGGTAG GGTTCATGGCCAAGGGTAAA 55.0 50.0 58.1 58.1 1041 bp 0.5 58.3
tRNA-Leu-tRNA-Pro_p4 TAACTCCGGGAAGGTGGTAG AAAGGAGGTTCATGGCCAAG 55.0 50.0 58.1 58.1 1047 bp 0.5 58.3
tRNA-Leu-tRNA-Pro_p5 CCAGAGACTTAACTCCGGGA GGTTCATGGCCAAGGGTAAA 55.0 50.0 58.2 58.1 1050 bp 0.5 58.2