Hotspot

tRNA-Met-tRNA-Leu

Genome · 55648-62177 · 6530 bp

Back to Onobrychis

Pi 0.233

MarkerSeek score 77.20

Primer available yes

Label tRNA-Met-tRNA-Leu

Feature Metadata

label_name: tRNA-Met-tRNA-Leu
feature_type: igs
region: Genome
length_bp: 6530
variable_sites: 2815
pi: 0.233
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Met-tRNA-Leu_p1	ACGCAATTCACAGGGTTCAA	ACTATTCCCGCAAGAGGTCT	45.0	50.0	58.3	58.1	805 bp	0.5	56.4
tRNA-Met-tRNA-Leu_p2	TTCACGCAATTCACAGGGTT	ACTATTCCCGCAAGAGGTCT	45.0	50.0	58.3	58.1	808 bp	0.5	56.4
tRNA-Met-tRNA-Leu_p3	TTTCACGCAATTCACAGGGT	ACTATTCCCGCAAGAGGTCT	45.0	50.0	58.3	58.1	809 bp	0.5	56.4
tRNA-Met-tRNA-Leu_p4	CTTTCACGCAATTCACAGGG	ACTATTCCCGCAAGAGGTCT	50.0	50.0	57.7	58.1	810 bp	0.5	56.2
tRNA-Met-tRNA-Leu_p5	ACGCAATTCACAGGGTTCAA	CCCGCAAGAGGTCTGTTTTA	45.0	50.0	58.3	57.8	799 bp	0.5	56.1