Hotspot

tRNA-Ile-tRNA-Ile

Genome · 46738-47697 · 960 bp

Back to Onobrychis

Pi 0.297

MarkerSeek score 71.90

Primer available yes

Label tRNA-Ile-tRNA-Ile

Feature Metadata

label_name: tRNA-Ile-tRNA-Ile
feature_type: igs
region: Genome
length_bp: 960
variable_sites: 427
pi: 0.297
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ile-tRNA-Ile_p1	AAAGTGAGCCACCAGTTCAG	CCTGTCCTCAGTTCGGATTG	50.0	55.0	58.0	58.0	1694-1695 bp	0.5	59.7
tRNA-Ile-tRNA-Ile_p2	TCTGACCAACTTCCCATCCT	CCTGTCCTCAGTTCGGATTG	50.0	55.0	58.0	58.0	1800-1801 bp	0.5	59.5
tRNA-Ile-tRNA-Ile_p3	TACCGTCCATGTACGATCCA	CCTGTCCTCAGTTCGGATTG	50.0	55.0	57.9	58.0	1827-1828 bp	0.5	59.4
tRNA-Ile-tRNA-Ile_p4	CGCACGACTCTTTGGTCTTA	CCTGTCCTCAGTTCGGATTG	50.0	55.0	57.9	58.0	2178-2179 bp	0.5	59.4
tRNA-Ile-tRNA-Ile_p5	AAAGTGAGCCACCAGTTCAG	CCTCAGTTCGGATTGTAGGC	50.0	55.0	58.0	58.1	1689-1690 bp	0.5	59.3