Hotspot

tRNA-Ala-tRNA-Ala

Genome · 45798-46600 · 803 bp

Back to Onobrychis

Pi 0.291

MarkerSeek score 72.00

Primer available yes

Label tRNA-Ala-tRNA-Ala

Feature Metadata

label_name: tRNA-Ala-tRNA-Ala
feature_type: igs
region: Genome
length_bp: 803
variable_sites: 353
pi: 0.291
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ala-tRNA-Ala_p1	ATGGAGATAAGCGGACTCGA	TGATTTACTTCACGGGCGAG	50.0	50.0	58.0	58.0	989 bp	0.5	59.8
tRNA-Ala-tRNA-Ala_p2	ATGTTTCAGTTCGCCAGGTT	TGATTTACTTCACGGGCGAG	45.0	50.0	58.0	58.0	1352 bp	0.5	59.8
tRNA-Ala-tRNA-Ala_p3	TTCTGGATGCTTCGCCTAAC	TGATTTACTTCACGGGCGAG	50.0	50.0	58.0	58.0	1487 bp	0.5	59.8
tRNA-Ala-tRNA-Ala_p4	ATGGAGATAAGCGGACTCGA	CAGGGTCTCTTAACGCCTTC	50.0	55.0	58.0	58.0	1183 bp	0.5	59.8
tRNA-Ala-tRNA-Ala_p5	TTTTCACCTTTCCCTCACGG	TGATTTACTTCACGGGCGAG	50.0	50.0	58.0	58.0	1660 bp	0.5	59.8