Hotspot

tRNA-Leu-tRNA-Asn

Genome · 25257-41312 · 16056 bp

Back to Onobrychis

Pi 0.264
MarkerSeek score 73.70
Primer available yes
Label tRNA-Leu-tRNA-Asn

Feature Metadata

label_name
tRNA-Leu-tRNA-Asn
feature_type
igs
region
Genome
length_bp
16056
variable_sites
6398
pi
0.264
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu-tRNA-Asn_p1 CTCGGACTGTTTGTTGACCA AATTCAGAACCGTCCGATCG 50.0 50.0 58.1 58.1 851 bp 0.5 59.0
tRNA-Leu-tRNA-Asn_p2 CTCGGACTGTTTGTTGACCA CAGAACCGTCCGATCGATTT 50.0 50.0 58.1 58.1 847 bp 0.5 59.0
tRNA-Leu-tRNA-Asn_p3 CTCGGACTGTTTGTTGACCA TTCGCTAGCGGAATGTGAAA 50.0 45.0 58.1 57.9 566 bp 0.5 58.9
tRNA-Leu-tRNA-Asn_p4 CTCGGACTGTTTGTTGACCA TTCCAGGAGCCGTATGAGAT 50.0 50.0 58.1 57.9 757 bp 0.5 58.9
tRNA-Leu-tRNA-Asn_p5 CTCGGACTGTTTGTTGACCA TTTCCAGGAGCCGTATGAGA 50.0 50.0 58.1 58.1 758 bp 0.5 58.5