Hotspot

tRNA-His-tRNA-Leu

Genome · 21359-25176 · 3818 bp

Back to Onobrychis

Pi 0.283

MarkerSeek score 77.50

Primer available yes

Label tRNA-His-tRNA-Leu

Feature Metadata

label_name: tRNA-His-tRNA-Leu
feature_type: igs
region: Genome
length_bp: 3818
variable_sites: 1630
pi: 0.283
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-His-tRNA-Leu_p1	ACTAGTTGCATGGCAGTTCC	TCAGTAGTCAATCGTCGAGA	50.0	45.0	58.2	55.5	740-742 bp	0.5	38.4
tRNA-His-tRNA-Leu_p2	AAAGTAGAAGTCCGACCCCT	TCAGTAGTCAATCGTCGAGA	50.0	45.0	57.7	55.5	1434-1436 bp	0.5	37.3
tRNA-His-tRNA-Leu_p3	ACTAGTTGCATGGCAGTTCC	TTCAGTAGTCAATCGTCGAGA	50.0	42.9	58.2	56.3	741-743 bp	0.5	36.7
tRNA-His-tRNA-Leu_p4	ACTAGTTGCATGGCAGTTCC	CCAATTCAGTAGTCAATCGTCG	50.0	45.5	58.2	57.2	745-747 bp	0.5	36.5
tRNA-His-tRNA-Leu_p5	AAAGTAGAAGTCCGACCCCT	TTCAGTAGTCAATCGTCGAGA	50.0	42.9	57.7	56.3	1435-1437 bp	0.5	35.6