Hotspot

tRNA-Met-tRNA-Lys

Genome · 12522-17242 · 4721 bp

Back to Onobrychis

Pi 0.29

MarkerSeek score 76.30

Primer available yes

Label tRNA-Met-tRNA-Lys

Feature Metadata

label_name: tRNA-Met-tRNA-Lys
feature_type: igs
region: Genome
length_bp: 4721
variable_sites: 2065
pi: 0.29
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Met-tRNA-Lys_p1	TGTCACTACCCTTCTCTGCA	TCGCCAGGGAAATATGTTGG	50.0	50.0	58.0	57.9	437 bp	0.5	59.5
tRNA-Met-tRNA-Lys_p2	TGTCACTACCCTTCTCTGCA	GGTTCGCCAGGGAAATATGT	50.0	50.0	58.0	57.9	440 bp	0.5	59.5
tRNA-Met-tRNA-Lys_p3	CATCGTTACCCATTGGTCGT	TCGCCAGGGAAATATGTTGG	50.0	50.0	58.0	57.9	358 bp	0.5	59.4
tRNA-Met-tRNA-Lys_p4	CATCGTTACCCATTGGTCGT	GGTTCGCCAGGGAAATATGT	50.0	50.0	58.0	57.9	361 bp	0.5	59.4
tRNA-Met-tRNA-Lys_p5	TGTCACTACCCTTCTCTGCA	GAGGGTTAGCTGCAAAAGGT	50.0	50.0	58.0	58.1	706 bp	0.5	59.2