Pi
0.27
MarkerSeek score
81.60
Primer available
yes
Label
tRNA-Thr-tRNA-Leu
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| tRNA-Thr-tRNA-Leu_p1 | CGAAGCCATACCCAATCGAA | TCTACCTGCTGAGCTATCCC | 50.0 | 55.0 | 58.1 | 58.0 | 2573-2598 bp | 0.5 | 59.6 |
| tRNA-Thr-tRNA-Leu_p2 | CGAAGCCATACCCAATCGAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1878-1883 bp | 0.5 | 59.6 |
| tRNA-Thr-tRNA-Leu_p3 | TTGACCCTTTCGCTTTTCCA | TCTACCTGCTGAGCTATCCC | 45.0 | 55.0 | 57.9 | 58.0 | 2502-2527 bp | 0.5 | 59.5 |
| tRNA-Thr-tRNA-Leu_p4 | TTGACCCTTTCGCTTTTCCA | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 57.9 | 58.0 | 1807-1812 bp | 0.5 | 59.5 |
| tRNA-Thr-tRNA-Leu_p5 | AGCGCGATTGGTTTCTAAGT | TCTACCTGCTGAGCTATCCC | 45.0 | 55.0 | 57.9 | 58.0 | 2379-2404 bp | 0.5 | 59.3 |