Hotspot

23S ribosomal RNA

Genome · 42776-45584 · 2809 bp

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Pi 0.26

MarkerSeek score 66.10

Primer available yes

Label 23S ribosomal RNA

Feature Metadata

label_name: 23S ribosomal RNA
feature_type: rRNA
region: Genome
length_bp: 2809
variable_sites: 1101
pi: 0.26
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
23S ribosomal RNA_p1	GCCGTGACCTTCTCTTGAAT	AGTAGCACCTTAGGATGGCA	50.0	50.0	57.9	58.1	2962 bp	0.5	58.2
23S ribosomal RNA_p2	ACTTCTTTTGCTCCATCCCC	AGTAGCACCTTAGGATGGCA	50.0	50.0	57.8	58.1	2935 bp	0.5	57.3
23S ribosomal RNA_p3	GCCGTGACCTTCTCTTGAAT	CCTTCAAGTTAAGGGCGAGG	50.0	55.0	57.9	58.3	2939 bp	0.5	57.1
23S ribosomal RNA_p4	ATTGCTGTCTCGGCTGTTAC	AGTAGCACCTTAGGATGGCA	50.0	50.0	58.3	58.1	2898 bp	0.5	56.9
23S ribosomal RNA_p5	CATTGCTGTCTCGGCTGTTA	AGTAGCACCTTAGGATGGCA	50.0	50.0	58.3	58.1	2899 bp	0.5	56.8