Hotspot

trnA-UGC

IRb · 102269-102323 · 55 bp

Back to Oncidium

Pi 0.037
MarkerSeek score 75.50
Primer available yes
Label trnA-UGC

Feature Metadata

label_name
trnA-UGC
feature_type
tRNA
region
IRb
length_bp
55
variable_sites
2
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC_p1 TGATTTACTTCACGGGCGAG TAGAAAAAGTGAGCCACCGG 50.0 50.0 58.0 57.8 239-240 bp 1.0 79.5
trnA-UGC_p2 GCGCCAGGGAAAAGAATAGA TAGAAAAAGTGAGCCACCGG 50.0 50.0 58.0 57.8 188-189 bp 1.0 79.5
trnA-UGC_p3 TGATTTACTTCACGGGCGAG CATGTTTCGGTCCTCTTCCC 50.0 55.0 58.0 58.3 266-267 bp 1.0 78.8
trnA-UGC_p4 AGGTCTCTGGTTCAAGTCCA TAGAAAAAGTGAGCCACCGG 50.0 50.0 57.9 57.8 221-222 bp 1.0 78.8
trnA-UGC_p5 GCGCCAGGGAAAAGAATAGA CATGTTTCGGTCCTCTTCCC 50.0 55.0 58.0 58.3 215-216 bp 1.0 78.7
trnA-UGC_p1 TGATTTACTTCACGGGCGAG ATCGAACTGCCGAATTGGAA 50.0 45.0 58.0 57.9 1037-1038 bp 1.0 79.3
trnA-UGC_p2 TGATTTACTTCACGGGCGAG TCGAACTGCCGAATTGGAAA 50.0 45.0 58.0 58.1 1036-1037 bp 1.0 79.3
trnA-UGC_p3 TGATTTACTTCACGGGCGAG GGTGCTGCTAAATGGAAGGA 50.0 50.0 58.0 57.9 1089-1090 bp 1.0 79.2
trnA-UGC_p4 TGATTTACTTCACGGGCGAG AGGTGCTGCTAAATGGAAGG 50.0 50.0 58.0 57.9 1090-1091 bp 1.0 79.1
trnA-UGC_p5 GCGCCAGGGAAAAGAATAGA ATCGAACTGCCGAATTGGAA 50.0 45.0 58.0 57.9 986-987 bp 1.0 79.1
trnA-UGC_p1 ATCGAACTGCCGAATTGGAA TGATTTACTTCACGGGCGAG 45.0 50.0 57.9 58.0 1037-1038 bp 1.0 79.3
trnA-UGC_p2 TCGAACTGCCGAATTGGAAA TGATTTACTTCACGGGCGAG 45.0 50.0 58.1 58.0 1036-1037 bp 1.0 79.3
trnA-UGC_p3 GGTGCTGCTAAATGGAAGGA TGATTTACTTCACGGGCGAG 50.0 50.0 57.9 58.0 1089-1090 bp 1.0 79.2
trnA-UGC_p4 AGGTGCTGCTAAATGGAAGG TGATTTACTTCACGGGCGAG 50.0 50.0 57.9 58.0 1090-1091 bp 1.0 79.1
trnA-UGC_p5 ATCGAACTGCCGAATTGGAA GCGCCAGGGAAAAGAATAGA 45.0 50.0 57.9 58.0 986-987 bp 1.0 79.1