Hotspot

rpoA

LSC · 76256-77275 · 1020 bp

Back to Oncidium

Pi 0.00294

MarkerSeek score 60.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 3
pi: 0.00294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGCAAATATATGGACCGTGTCA	GGATGTCGTCCCCCTAAAAA	40.9	50.0	57.9	57.2	1219 bp	1.0	56.7
rpoA_p2	TCTGCAAATATATGGACCGTGT	GGATGTCGTCCCCCTAAAAA	40.9	50.0	57.6	57.2	1221 bp	1.0	54.5
rpoA_p3	CTGCAAATATATGGACCGTGTC	GGATGTCGTCCCCCTAAAAA	45.5	50.0	57.3	57.2	1220 bp	1.0	52.0
rpoA_p4	GCAAATATATGGACCGTGTCA	GGATGTCGTCCCCCTAAAAA	42.9	50.0	56.1	57.2	1218 bp	1.0	50.8
rpoA_p5	TGCAAATATATGGACCGTGTC	GGATGTCGTCCCCCTAAAAA	42.9	50.0	56.1	57.2	1219 bp	1.0	50.8