Hotspot

rps15-trnN-GUU

SSC · 115218-121266 · 6049 bp

Back to Oncidium

Pi 0.00678

MarkerSeek score 56.40

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 6049
variable_sites: 41
pi: 0.00678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	AGTAACCTCTCCCTCTTCCG	AGAATGCCGTCGCCTATTTT	55.0	45.0	57.9	58.0	1297-1299 bp	1.0	80.1
rps15-trnN-GUU_p2	GTAACCTCTCCCTCTTCCGT	AGAATGCCGTCGCCTATTTT	55.0	45.0	58.2	58.0	1296-1298 bp	1.0	80.0
rps15-trnN-GUU_p3	GGATCAAGATTGCCCGTGAT	AGAATGCCGTCGCCTATTTT	50.0	45.0	58.0	58.0	850 bp	0.5	59.5
rps15-trnN-GUU_p4	GGATCAAGATTGCCCGTGAT	CCTCAGAAACGGAAGAAGGG	50.0	55.0	58.0	57.9	804 bp	0.5	59.0
rps15-trnN-GUU_p5	GGATCAAGATTGCCCGTGAT	CTCAGAAACGGAAGAAGGGG	50.0	55.0	58.0	57.9	803 bp	0.5	59.0