Hotspot

rps12-clpP

LSC · 67679-67804 · 126 bp

Back to Oncidium

Pi 0.0397

MarkerSeek score 60.30

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 5
pi: 0.0397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	ATTGGCTGTCTTGCATTTCT	GCAGTCGAAAATGAGAATACTGG	40.0	43.5	55.9	57.9	203-213 bp	1.0	42.3
rps12-clpP_p2	ATTGGCTGTCTTGCATTTCT	TGTAGCAGTCGAAAATGAGA	40.0	40.0	55.9	54.4	207-217 bp	1.0	42.3
rps12-clpP_p3	ATTGGCTGTCTTGCATTTCT	TCTTGTAGCAGTCGAAAATGAGA	40.0	39.1	55.9	57.9	210-220 bp	1.0	42.2
rps12-clpP_p4	ATTGGCTGTCTTGCATTTCT	CTTGTAGCAGTCGAAAATGAGA	40.0	40.9	55.9	56.5	209-219 bp	1.0	42.2
rps12-clpP_p5	ATTGGCTGTCTTGCATTTCT	TCTTGTAGCAGTCGAAAATGAG	40.0	40.9	55.9	56.5	210-220 bp	1.0	42.2