Hotspot

psbE-petL

LSC · 63004-63888 · 885 bp

Back to Oncidium

Pi 0.091

MarkerSeek score 96.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 77
pi: 0.091
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 96.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.9	58.0	1042-1185 bp	1.0	79.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1038-1181 bp	1.0	79.9
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1040-1183 bp	1.0	79.9
psbE-petL_p4	ACTGACGAATAACCAACCCG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1051-1194 bp	1.0	79.6
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1053-1196 bp	1.0	79.5