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trnF-GAA-ndhJ

LSC · 47724-48325 · 602 bp

Back to Oncidium

Pi 0.0183

MarkerSeek score 61.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 11
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCTATGATGTAGCACCAGGC	50.0	55.0	58.1	57.8	709 bp	1.0	79.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GCCTATGATGTAGCACCAGG	50.0	55.0	58.1	57.8	710 bp	1.0	79.4
trnF-GAA-ndhJ_p3	TATGCTAGGATGATGCACGC	CCTATGATGTAGCACCAGGC	50.0	55.0	57.9	57.8	774 bp	1.0	79.1
trnF-GAA-ndhJ_p4	TATGCTAGGATGATGCACGC	GCCTATGATGTAGCACCAGG	50.0	55.0	57.9	57.8	775 bp	1.0	79.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CTCCCAGTGTGCCTATGATG	50.0	55.0	58.1	57.7	720 bp	1.0	78.8