Hotspot

psbM-trnD-GUC

LSC · 28908-30043 · 1136 bp

Back to Oncidium

Pi 0.0132

MarkerSeek score 55.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 15
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1268-1295 bp	1.0	78.5
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1246-1273 bp	1.0	78.3
psbM-trnD-GUC_p3	TGAACAGCGCAGTAGCAATA	CCATGCATATGGCTGGGATT	45.0	50.0	57.6	58.1	1267-1294 bp	1.0	77.5
psbM-trnD-GUC_p4	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1245-1272 bp	1.0	77.4
psbM-trnD-GUC_p5	AGCGCAGTAGCAATAAGAGC	CCATGCATATGGCTGGGATT	50.0	50.0	58.4	58.1	1262-1289 bp	1.0	77.1