Hotspot

atpA-atpF

LSC · 11474-11545 · 72 bp

Back to Oncidium

Pi 0.0278

MarkerSeek score 68.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 72
variable_sites: 2
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACTTGAAGTACGGTACCGG	ATTGGTATCCTCAGGTCCGT	55.0	50.0	58.0	57.8	216 bp	1.0	79.2
atpA-atpF_p2	CCACTTGAAGTACGGTACCG	ATTGGTATCCTCAGGTCCGT	55.0	50.0	58.0	57.8	217 bp	1.0	79.2
atpA-atpF_p3	CACTTGAAGTACGGTACCGG	TATCCTCAGGTCCGTGGAAG	55.0	55.0	58.0	58.2	211 bp	1.0	78.9
atpA-atpF_p4	CCACTTGAAGTACGGTACCG	TATCCTCAGGTCCGTGGAAG	55.0	55.0	58.0	58.2	212 bp	1.0	78.9
atpA-atpF_p5	GCCCACTTGAAGTACGGTAC	ATTGGTATCCTCAGGTCCGT	55.0	50.0	58.3	57.8	219 bp	1.0	77.1