Hotspot

ycf1

SSC · 123569-129142 · 5574 bp

Back to Omphalogramma

Pi 0.0147

MarkerSeek score 51.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5574
variable_sites: 212
pi: 0.0147
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTGCGCATATGGGATCGT	TCCTTTCTTGAATGGGCCAC	45.0	50.0	58.1	58.1	906-921 bp	0.833333	76.3
ycf1_p2	TCTTCACCCTCTCCTTCCTG	CACATCGCGGACAAATCCTA	55.0	50.0	58.1	58.1	436-445 bp	0.833333	75.5
ycf1_p3	TCTTCACCCTCTCCTTCCTG	TCCTTTCTTGAATGGGCCAC	55.0	50.0	58.1	58.1	453-462 bp	0.833333	75.3
ycf1_p4	TTCACCCTCTCCTTCCTGTT	CACATCGCGGACAAATCCTA	50.0	50.0	57.9	58.1	434-443 bp	0.833333	75.0
ycf1_p5	ATTTGCGCATATGGGATCGT	CACATCGCGGACAAATCCTA	45.0	50.0	58.1	58.1	889-904 bp	0.666667	67.9