Hotspot

trnV-GAC-rps12

IRa · 137853-139343 · 1491 bp

Back to Omphalogramma

Pi 0.00349
MarkerSeek score 62.90
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1491
variable_sites
13
pi
0.00349
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GGATAACCAGGCTCGAACTG TGGCAACTTTACGTAAGGCA 55.0 45.0 58.1 57.7 1594-1629 bp 1.0 79.0
trnV-GAC-rps12_p2 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1575-1610 bp 1.0 78.2
trnV-GAC-rps12_p3 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1579-1614 bp 1.0 78.1
trnV-GAC-rps12_p4 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1574-1609 bp 1.0 78.1
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1577-1612 bp 1.0 77.2