Hotspot

ccsA-ndhD

SSC · 115143-115392 · 250 bp

Back to Omphalogramma

Pi 0.0278

MarkerSeek score 62.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 250
variable_sites: 16
pi: 0.0278
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCCAATTCTTCGATTGTGGC	TATCTTGACGAAATGGGCGG	50.0	50.0	58.1	58.1	798-806 bp	1.0	83.3
ccsA-ndhD_p2	ACCTGCTCTCCAATCTGAGT	TATCTTGACGAAATGGGCGG	50.0	50.0	58.1	58.1	1267-1275 bp	1.0	83.1
ccsA-ndhD_p3	TGGTATTGGGTTATGCAGCC	TATCTTGACGAAATGGGCGG	50.0	50.0	57.9	58.1	1223-1231 bp	1.0	83.1
ccsA-ndhD_p4	ACCTGCTCTCCAATCTGAGT	TGGTGCTGCACTCTTTTTCT	50.0	45.0	58.1	57.9	1322-1330 bp	1.0	82.9
ccsA-ndhD_p5	TGGTATTGGGTTATGCAGCC	TGGTGCTGCACTCTTTTTCT	50.0	45.0	57.9	57.9	1278-1286 bp	1.0	82.9