Hotspot

rpl36-rps8

LSC · 80228-80685 · 458 bp

Back to Omphalogramma

Pi 0.0226

MarkerSeek score 70.40

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 28
pi: 0.0226
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ACTATAATTCGTCCCCGCCT	AATTTTAGGCGGAATGGGGG	50.0	50.0	58.3	58.2	642-650 bp	1.0	79.7
rpl36-rps8_p2	ACTATAATTCGTCCCCGCCT	GCGGAATGGGGGTTGTAATT	50.0	50.0	58.3	58.5	634-642 bp	1.0	77.3
rpl36-rps8_p3	ACTATAATTCGTCCCCGCCT	CGGAATGGGGGTTGTAATTCT	50.0	47.6	58.3	58.0	633-641 bp	1.0	73.4
rpl36-rps8_p4	ACTATAATTCGTCCCCGCCTA	AATTTTAGGCGGAATGGGGG	47.6	50.0	58.1	58.2	642-650 bp	1.0	73.1
rpl36-rps8_p5	TACTATAATTCGTCCCCGCCT	AATTTTAGGCGGAATGGGGG	47.6	50.0	58.1	58.2	643-651 bp	1.0	73.1