Hotspot

ndhC-trnV-UAC

LSC · 50741-51861 · 1121 bp

Back to Omphalogramma

Pi 0.0165
MarkerSeek score 63.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1121
variable_sites
44
pi
0.0165
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCATAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.2 57.8 990-1192 bp 1.0 47.1
ndhC-trnV-UAC_p2 TCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 26.1 50.0 52.2 57.8 989-1191 bp 1.0 47.1
ndhC-trnV-UAC_p3 ATCATATTCATAAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.2 57.5 989-1191 bp 1.0 47.1
ndhC-trnV-UAC_p4 TCATATTCATAAAGCAGAAACAT GAAGGTCTACGGTTCGAGTC 26.1 55.0 52.2 57.5 988-1190 bp 1.0 47.1
ndhC-trnV-UAC_p5 ATCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.9 57.8 990-1192 bp 1.0 47.1