Hotspot

trnF-GAA-ndhJ

LSC · 48381-49054 · 674 bp

Back to Omphalogramma

Pi 0.0141

MarkerSeek score 67.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 22
pi: 0.0141
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	782-830 bp	1.0	85.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	746-794 bp	1.0	85.1
trnF-GAA-ndhJ_p3	TCAAATCTGGTTCCTGGCAC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	731-779 bp	1.0	84.9
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	770-818 bp	1.0	84.4
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGATAATCATCCGCGCTTGA	55.0	45.0	58.0	57.5	822-870 bp	1.0	81.8