Hotspot

petN-psbM

LSC · 28577-29584 · 1008 bp

Back to Omphalogramma

Pi 0.0155

MarkerSeek score 61.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 42
pi: 0.0155
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGCTCAATTATTCCCCAGCA	TTTTCGTTTGATTCGGCGTG	50.0	45.0	57.9	58.0	1804-1844 bp	1.0	84.7
petN-psbM_p2	TGGCTCAATTATTCCCCAGC	TTTTCGTTTGATTCGGCGTG	50.0	45.0	57.9	58.0	1805-1845 bp	1.0	84.7
petN-psbM_p3	GGCTCAATTATTCCCCAGCA	ATCGGGAATCCCACTTTTCG	50.0	50.0	57.9	58.0	1818-1858 bp	1.0	84.5
petN-psbM_p4	AAGGGGCGGAATTTCGATAC	TTTTCGTTTGATTCGGCGTG	50.0	45.0	58.0	58.0	1653-1684 bp	0.833333	75.9
petN-psbM_p5	AAGGGGCGGAATTTCGATAC	ATCGGGAATCCCACTTTTCG	50.0	50.0	58.0	58.0	1667-1698 bp	0.833333	75.7